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pET28a-MGGG-ybbr-HIS-SdrD_B1-DK
(Plasmid #117983)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 117983 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5216
  • Total vector size (bp) 5720
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SdrG_B2
  • Alt name
    Staphylococcus aureus SD-repeat protein D
  • Species
    Staphylococcus aureus
  • Insert Size (bp)
    504
  • GenBank ID
    WP_000934487
  • Promoter T7
  • Tags / Fusion Proteins
    • HIS (N terminal on insert)
    • ybbr (N terminal on insert)
    • sortase (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer CTAGTTATTGCTCAGCGGT
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-MGGG-ybbr-HIS-SdrD_B1-DK was a gift from Hermann Gaub (Addgene plasmid # 117983 ; http://n2t.net/addgene:117983 ; RRID:Addgene_117983)
  • For your References section:

    Calcium stabilizes the strongest protein fold. Milles LF, Unterauer EM, Nicolaus T, Gaub HE. Nat Commun. 2018 Nov 12;9(1):4764. doi: 10.1038/s41467-018-07145-6. 10.1038/s41467-018-07145-6 PubMed 30420680