hCMV-IE1:NLuc
(Plasmid
#118065)
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PurposeTranscriptional unit for constitutive expression of NLuc in mammalian cells
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Depositing Lab
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Depositing OrganizationBaylor College of Medicine
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 118065 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 118065-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 118065-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneColE1 vector Alpha2
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Backbone manufacturerSelf made; Derived from pBluescriptSK
- Backbone size w/o insert (bp) 2327
- Total vector size (bp) 3677
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Modifications to backboneKanamycin Resistance
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Vector typeMammalian Expression, Luciferase, Synthetic Biology ; Assembly of GB2.0 transcriptional units
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCMV:NLuc:bGH
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Alt nameCytomegalovirus enhancer and promoter driving the expression of the NLuc Luciferase
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SpeciesSynthetic
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Insert Size (bp)2517
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
- 5′ sequencing primer aat gag ctg att taa caa aaa ttt aac gcg
- 3′ sequencing primer ctt ttt acg gtt cct ggc ctt ttg
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert can be released with BsmBI
DNA (Catalog # 118065-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 118065-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hCMV-IE1:NLuc was a gift from Koen Venken (Addgene plasmid # 118065 ; http://n2t.net/addgene:118065 ; RRID:Addgene_118065) -
For your References section:
Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying. Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young DW, Venken KJT. Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y. 10.1038/s41467-019-13651-y PubMed 31836712
