PurposeFor cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||118221||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typezebrafish expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Copy numberHigh Copy
Gene/Insert nameBrainbow 1.0 L - ccdB survival cassette
SpeciesFluorescent protein genes from jellyfish and coral
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer mCherry-R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Zebrabow-GateDest was a gift from Albert Pan & Alex Schier (Addgene plasmid # 118221 ; http://n2t.net/addgene:118221 ; RRID:Addgene_118221)
For your References section:Zebrabow: multispectral cell labeling for cell tracing and lineage analysis in zebrafish. Pan YA, Freundlich T, Weissman TA, Schoppik D, Wang XC, Zimmerman S, Ciruna B, Sanes JR, Lichtman JW, Schier AF. Development. 2013 Jul;140(13):2835-46. doi: 10.1242/dev.094631. 10.1242/dev.094631 PubMed 23757414