WT MetNI in pBAD
(Plasmid #118254)

• Purpose: Wild Type Methionine ABC Transporter MetNI in pBAD Vector for Transport Activity Assay
• Depositing Lab: Douglas Rees
• Publication: Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. pii: 1811003115. doi: 10.1073/pnas.1811003115.

Backbone

• Vector backbone: pBAD
• Backbone manufacturer: Thermo Scientific Invitrogen
• Backbone size w/o insert (bp): 3966
• Total vector size (bp): 5713
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: MetN
• Species: Escherichia coli
• Insert Size (bp): 1032
• GenBank ID: 944896
• Entrez Gene: metN (a.k.a. b0199, ECK0199, JW0195, abc, metD)
• Promoter: Arabinose Promoter
• Tags / Fusion Proteins:
  • 10x His Tag (N terminal on insert)
  • Enterokinase Cleavage Site (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: pBAD Forward
• 3′ sequencing primer: ttagcaataatttgccccggacgcgtgacata

Gene/Insert 2

• Gene/Insert name: MetI
• Species: Escherichia coli
• Insert Size (bp): 654
• GenBank ID: 944894
• Entrez Gene: metI (a.k.a. b0198, ECK0198, JW0194, metD, yaeE)
• Promoter: None

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: tagcgcgcagatggattacgccggtggcgttaagttcggc
• 3′ sequencing primer: pBAD Reverse

Resource Information

• Supplemental Documents:
  • wt-metni-in-pbad.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry