GFP-Rap1b S179AS180A
(Plasmid
#118327)
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PurposeExpression of bovine Rap1b
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Depositing Lab
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Depositing OrganizationOregon Health & Science University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 118327 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 118327-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 118327-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepeGFP-C1
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Backbone manufacturerclontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRap1b S179AS180A
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SpeciesB. taurus (bovine)
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Insert Size (bp)555
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MutationS179AS180A
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Entrez GeneRAP1B
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site ApaI (unknown if destroyed)
- 5′ sequencing primer EGFP-C
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 118327-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 118327-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-Rap1b S179AS180A was a gift from Philip Stork (Addgene plasmid # 118327 ; http://n2t.net/addgene:118327 ; RRID:Addgene_118327) -
For your References section:
Protein kinase A-dependent phosphorylation of Rap1 regulates its membrane localization and cell migration. Takahashi M, Dillon TJ, Liu C, Kariya Y, Wang Z, Stork PJ. J Biol Chem. 2013 Sep 27;288(39):27712-23. doi: 10.1074/jbc.M113.466904. Epub 2013 Aug 14. 10.1074/jbc.M113.466904 PubMed 23946483
