pMCh1807_pBS_cdc42E7-boxB
(Plasmid #118612)

• Purpose: cdc42E7 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown
• Depositing Lab: Marina Chekulaeva
• Publication: Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 5258023. doi: 10.1093/nar/gky1270.

Backbone

• Vector backbone: pBS
• Backbone size w/o insert (bp): 3311
• Total vector size (bp): 4724
• Vector type: in vitro transcription

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cdc42E7 3'UTR
• Species: M. musculus (mouse)
• Insert Size (bp): 1409
• GenBank ID: NM_009861 NM_009861
• Entrez Gene: Cdc42
• Promoter: T3
• Tag / Fusion Protein:
  • boxB (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: caggaaacagctatgac
• 3′ sequencing primer: gatccactagttctaga

Resource Information

• Supplemental Documents:
  • pMCh1807_pBS_cdc42E7-boxB
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Addgene NGS unable to fully resolve the poly-A tail length. Please refer to the depositor's sequence for this section of the plasmid.