pDONR 221-AIP2 (NON-STOP)
(Plasmid
#118678)
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PurposeGateway entry vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 118678 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR 221
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Vector typeOther
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAIP2
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)933
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Entrez GeneAT5G20910 (a.k.a. F22D1.80, F22D1_80)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications:
Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR 221-AIP2 (NON-STOP) was a gift from Kristiina Himanen (Addgene plasmid # 118678 ; http://n2t.net/addgene:118678 ; RRID:Addgene_118678) -
For your References section:
Genomic and Phenomic Screens for Flower Related RING Type Ubiquitin E3 Ligases in Arabidopsis. Pavicic M, Mouhu K, Wang F, Bilicka M, Chovancek E, Himanen K. Front Plant Sci. 2017 Mar 28;8:416. doi: 10.3389/fpls.2017.00416. eCollection 2017. 10.3389/fpls.2017.00416 PubMed 28400782