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pCAG-Scarlet-Kif5a
(Plasmid #118745)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 118745 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Clontech C4
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5957
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kif5a cDNA
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3106
  • Entrez Gene
    Kif5a (a.k.a. D10Bwg0738e, Khc, Kif5, Kns, mKIAA4086)
  • Promoter CAG
  • Tag / Fusion Protein
    • mScarlet (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer DSRed1-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-Scarlet-Kif5a was a gift from Beverly Koller (Addgene plasmid # 118745 ; http://n2t.net/addgene:118745 ; RRID:Addgene_118745)