pQLinkHD_LSSmOrange
(Plasmid
#118860)
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Purposefluorescent protein expression with an N-terminal His-tag
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 118860 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepQLinkHD
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BL21
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Growth instructionsUse BL21 for protein expression.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLSSmOrange
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MutationA44V,F84L, W145M, I165D, M167L, G202D substitutions
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Tag
/ Fusion Protein
- His-tag (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pQE_F CCCGAAAAGTGCCACCTG
- 3′ sequencing primer pQE_R GTTCTGAGGTCATTACTGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQLinkHD_LSSmOrange was a gift from Marc Birtwistle (Addgene plasmid # 118860 ; http://n2t.net/addgene:118860 ; RRID:Addgene_118860) -
For your References section:
Fluorescence Multiplexing with Spectral Imaging and Combinatorics. Holzapfel HY, Stern AD, Bouhaddou M, Anglin CM, Putur D, Comer S, Birtwistle MR. ACS Comb Sci. 2018 Nov 12;20(11):653-659. doi: 10.1021/acscombsci.8b00101. Epub 2018 Oct 30. 10.1021/acscombsci.8b00101 PubMed 30339749
