ID1-pLVXT
(Plasmid
#119280)
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PurposeDoxycycline-inducible mouse ID1 from 806 cells
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Depositing Lab
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Depositing OrganizationMemorial Sloan-Kettering Cancer Center
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119280 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 119280-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 119280-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepLVX-tight-Puro
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 7800
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameId1
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SpeciesM. musculus (mouse)
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Insert Size (bp)447
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GenBank ID
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Entrez GeneId1 (a.k.a. AI323524, D2Wsu140e, Idb1, bHLHb24)
- Promoter TRE
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer pLVXT
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 119280-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 119280-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ID1-pLVXT was a gift from Joan Massague (Addgene plasmid # 119280 ; http://n2t.net/addgene:119280 ; RRID:Addgene_119280) -
For your References section:
ID1 mediates escape from TGF-beta tumor suppression in pancreatic cancer. Huang YH, Hu J, Chen F, Lecomte N, Basnet H, David CJ, Witkin MD, Allen PJ, Leach SD, Hollmann TJ, Iacobuzio-Donahue CA, Massague J. Cancer Discov. 2019 Oct 3. pii: 2159-8290.CD-19-0529. doi: 10.1158/2159-8290.CD-19-0529. 10.1158/2159-8290.CD-19-0529 PubMed 31582374
Map uploaded by the depositor.
