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pcDNA3.1/HisC 14-3-3 epsilon K49E
(Plasmid #11943)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 11943 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1 His C
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5514
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ywhae
  • Alt name
    14-3-3 epsilon
  • Species
    R. norvegicus (rat)
  • Mutation
    K49E
  • Entrez Gene
    Ywhae (a.k.a. 14-3-3e)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1/HisC 14-3-3 epsilon K49E was a gift from Michael Yaffe (Addgene plasmid # 11943 ; http://n2t.net/addgene:11943 ; RRID:Addgene_11943)
  • For your References section:

    14-3-3 transits to the nucleus and participates in dynamic nucleocytoplasmic transport. Brunet A, Kanai F, Stehn J, Xu J, Sarbassova D, Frangioni JV, Dalal SN, DeCaprio JA, Greenberg ME, Yaffe MB. J Cell Biol. 2002 Mar 4. 156(5):817-28. 10.1083/jcb.200112059 PubMed 11864996