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pBMN mCherry-NBR1(F562A/Y731A/ΔUBD)
(Plasmid #119692)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119692 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBMN
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NBR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2715
  • Mutation
    F562A/Y731A & UBD deletion (1-906 ), See depositor comments below
  • Entrez Gene
    NBR1 (a.k.a. 1A1-3B, IAI3B, M17S2, MIG19)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please Note: The Phenylalanine to Alanine mutation is at position 563, and the Tyrosine to Alanine mutation is at position 732 relative to the NCBI Reference Sequence NP_005890.2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBMN mCherry-NBR1(F562A/Y731A/ΔUBD) was a gift from Michael Lazarou (Addgene plasmid # 119692 ; http://n2t.net/addgene:119692 ; RRID:Addgene_119692)

  • For your References section:

    LC3/GABARAPs drive ubiquitin-independent recruitment of Optineurin and NDP52 to amplify mitophagy. Padman BS, Nguyen TN, Uoselis L, Skulsuppaisarn M, Nguyen LK, Lazarou M. Nat Commun. 2019 Jan 24;10(1):408. doi: 10.1038/s41467-019-08335-6. 10.1038/s41467-019-08335-6 PubMed 30679426
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