pGRE NPD-GFP
(Plasmid #1197)

• Depositing Lab: Susan Lindquist
• Publication: Patino et al Science 1996 Aug 2;273(5275):622-6.

Backbone

• Vector backbone: pUC18 w/ GRE promoter
• Backbone size w/o insert (bp): 2800
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: N-terminus of Sup35 fused to GFP
• Alt name: Sup35
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1200
• Mutation: fused N-terminal Sup35 (~1-253aa) to GFP
• GenBank ID: NC_001136
• Entrez Gene: SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
• Tag / Fusion Protein:
  • GFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: M13/pUC Forward
• 3′ sequencing primer: M13 Rev

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

2 micron yeast plasmid

Please note that BamHI and SpeI are NOT single cutters in this plasmid. See the Addgene digest above.