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(Plasmid #1197)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 1197 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pUC18 w/ GRE promoter
  • Backbone size w/o insert (bp) 2800
  • Vector type
    Yeast Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    N-terminus of Sup35 fused to GFP
  • Alt name
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
  • Mutation
    fused N-terminal Sup35 (~1-253aa) to GFP
  • GenBank ID
  • Entrez Gene
    SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
  • Tag / Fusion Protein
    • GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer M13/pUC Forward
  • 3′ sequencing primer M13 Rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

2 micron yeast plasmid

Please note that BamHI and SpeI are NOT single cutters in this plasmid. See the Addgene digest above.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGRE NPD-GFP was a gift from Susan Lindquist (Addgene plasmid # 1197 ; ; RRID:Addgene_1197)
  • For your References section:

    Support for the prion hypothesis for inheritance of a phenotypic trait in yeast. Patino MM, Liu JJ, Glover JR, Lindquist S. Science 1996 Aug 2;273(5275):622-6. 10.1126/science.273.5275.622 PubMed 8662547