PurposeConversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||119770||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4919
Vector typePlant Expression
Growth in Bacteria
Copy numberHigh Copy
MutationnCas9 has a mutation D10A in wild type SpCas9
- Promoter maize Ubiquitin-1
/ Fusion Protein
- NLS (C terminal on insert)
- Cloning method Gibson Cloning
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:A3A-PBE-ΔUGI was a gift from Caixia Gao (Addgene plasmid # 119770 ; http://n2t.net/addgene:119770 ; RRID:Addgene_119770)
For your References section:Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. Zong Y, Song Q, Li C, Jin S, Zhang D, Wang Y, Qiu JL, Gao C. Nat Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261. 10.1038/nbt.4261 PubMed 30272679