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pCAGGS-FuG-E (P440E)
(Plasmid #119978)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 119978 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

This item is currently unavailable outside the US without additional regulatory approval.
A non-refundable shipping export licensing fee of $85 is required to cover Addgene’s additional processing costs.

Backbone

  • Vector backbone
    pCAGGS vector
  • Backbone size w/o insert (bp) 4900
  • Total vector size (bp) 6406
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Stbl3 and DH5alpha are also suitable growth strains.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fusion Glycoprotein type E (P440E)
  • Alt name
    FuG-E (P440E)
  • Species
    G. gallus (chicken), B. taurus (bovine); Vesicular stomatitis Indiana virus, Rabies virus, Cytomegalovirus, Simian virus 40, Oryctolagus cuniculus
  • Insert Size (bp)
    1515
  • Promoter CAGGS
  • Tag / Fusion Protein
    • Fusion protein of Vesicular stomatitis Indiana virus and Rabies virus (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS was unable to fully resolve the sequence of the CAGGS promoter. Please refer to the depositor's sequence for this section of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-FuG-E (P440E) was a gift from Kazuto Kobayashi (Addgene plasmid # 119978 ; http://n2t.net/addgene:119978 ; RRID:Addgene_119978)
  • For your References section:

    Enhancement of the transduction efficiency of a lentiviral vector for neuron-specific retrograde gene delivery through the point mutation of fusion glycoprotein type E. Kato S, Sugawara M, Kobayashi K, Kimura K, Inoue KI, Takada M, Kobayashi K. J Neurosci Methods. 2018 Oct 19;311:147-155. doi: 10.1016/j.jneumeth.2018.10.023. 10.1016/j.jneumeth.2018.10.023 PubMed 30347222