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(Plasmid #120088)


Item Catalog # Description Quantity Price (USD)
Plasmid 120088 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 7032
  • Modifications to backbone
    Plasmid assembled from 4 CYANO-VECTOR modular devices: pCVD048 / CV-pANS: pANS is a replication device carrying a fragment of pANS (the small plasmid of Synechococcus elongatus PCC7942) including repA and repB for replication of the plasmid in S. elongatus. pCVD026 / CV-oribom: oribom is a replication device for E. coli with an origin and a bom site, for conjugal transfer, from pBR322 to assembled with a cyanobacterial replicon. pCVD002 / CV-aadA: aadA is a device that confers spectinomycin and streptomycin (SpSm) resistance. The stop codon was erased by mistake, another stop codon is however located 15 nt downstream in the transcriptional terminator. This device works perfectly well. pCVD031 / PconII-LTRBS-yemGFP: PconII-LTRBS-yemGFP is a functional device where yemGFP is driven by a PconII promoter; RBS and flanking sequences from the pENTR/SD/D-Topo vector.
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Plasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
  • Species
    Synechococcus elongatus PCC 7942

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM4787 was a gift from Susan Golden (Addgene plasmid # 120088 ; ; RRID:Addgene_120088)
  • For your References section:

    Self-replicating shuttle vectors based on pANS, a small endogenous plasmid of the unicellular cyanobacterium Synechococcus elongatus PCC 7942. Chen Y, Taton A, Go M, London RE, Pieper LM, Golden SS, Golden JW. Microbiology. 2016 Dec;162(12):2029-2041. doi: 10.1099/mic.0.000377. Epub 2016 Oct 14. 10.1099/mic.0.000377 PubMed 27902432