pAM4787
(Plasmid #120088)

• Purpose: Cyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).
• Depositing Lab: Susan Golden
• Depositing Organization: University of California, San Diego (UCSD)
  Located in the United States of America
• Publication: Chen et al Microbiology. 2016 Dec;162(12):2029-2041. doi: 10.1099/mic.0.000377. Epub 2016 Oct 14.

Backbone

• Vector backbone: pBR322-aadA
• Backbone size w/o insert (bp): 7032
• Modifications to backbone: Plasmid assembled from 4 CYANO-VECTOR modular devices: pCVD048 / CV-pANS: pANS is a replication device carrying a fragment of pANS (the small plasmid of Synechococcus elongatus PCC7942) including repA and repB for replication of the plasmid in S. elongatus. pCVD026 / CV-oribom: oribom is a replication device for E. coli with an origin and a bom site, for conjugal transfer, from pBR322 to assembled with a cyanobacterial replicon. pCVD002 / CV-aadA: aadA is a device that confers spectinomycin and streptomycin (SpSm) resistance. The stop codon was erased by mistake, another stop codon is however located 15 nt downstream in the transcriptional terminator. This device works perfectly well. pCVD031 / PconII-LTRBS-yemGFP: PconII-LTRBS-yemGFP is a functional device where yemGFP is driven by a PconII promoter; RBS and flanking sequences from the pENTR/SD/D-Topo vector.
• Vector type: other

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin and Streptomycin, 50 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Plasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
• Species: Synechococcus elongatus PCC 7942

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 120088-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 120088-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.