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pLV-EF1a-V5-LIC
(Plasmid #120247)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 120247 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLV-EF1a-IRES-Puro
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin
  • Tag / Fusion Protein
    • V5 (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • V5 (N terminal on backbone)

Resource Information

Depositor Comments

For LIC cloning: cut with SrfI before T4 polymerase treatment

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-EF1a-V5-LIC was a gift from Larry Gerace (Addgene plasmid # 120247 ; http://n2t.net/addgene:120247 ; RRID:Addgene_120247)