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pRK5 FLAG membrane-bound lipin-1 (TMD)
(Plasmid #120277)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120277 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 120277-D50 50 μg of DNA in Tris buffer $495
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DNA 120277-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lipin-1
  • Alt name
    phosphatidate phosphatase 1
  • Alt name
    fatty liver dystrophy
  • Alt name
    PAP1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2900
  • Entrez Gene
    Lpin1 (a.k.a. Lipin1, fld)
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • in-frame fusion with the TMD Golgi-localization domain of Syntaxin5 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    David Sabatini Addgene Plasmid #32005

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5 FLAG membrane-bound lipin-1 (TMD) was a gift from Sirio Dupont (Addgene plasmid # 120277 ; http://n2t.net/addgene:120277 ; RRID:Addgene_120277)

  • For your References section:

    Extracellular matrix mechanical cues regulate lipid metabolism through Lipin-1 and SREBP. Romani P, Brian I, Santinon G, Pocaterra A, Audano M, Pedretti S, Mathieu S, Forcato M, Bicciato S, Manneville JB, Mitro N, Dupont S. Nat Cell Biol. 2019 Mar;21(3):338-347. doi: 10.1038/s41556-018-0270-5. Epub 2019 Feb 4. 10.1038/s41556-018-0270-5 PubMed 30718857
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