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pSYN-VWF-039
(Plasmid #120292)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120292 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 120292-D50 50 μg of DNA in Tris buffer $495
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DNA 120292-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5011
  • Total vector size (bp) 9503
  • Modifications to backbone
    -
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VWF D1-D2-D'-D3-Fc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4482
  • Mutation
    C1099A/C1142A
  • Promoter CMV
  • Tag / Fusion Protein
    • Fc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsiWI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSYN-VWF-039 was a gift from Robert Peters (Addgene plasmid # 120292 ; http://n2t.net/addgene:120292 ; RRID:Addgene_120292)

  • For your References section:

    The von Willebrand factor D'D3 assembly and structural principles for factor VIII binding and concatemer biogenesis. Dong X, Leksa NC, Chhabra ES, Arndt JW, Lu Q, Knockenhauer KE, Peters RT, Springer TA. Blood. 2019 Jan 14. pii: blood-2018-10-876300. doi: 10.1182/blood-2018-10-876300. 10.1182/blood-2018-10-876300 PubMed 30642920
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