Purpose(Empty Backbone) To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||120308||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5891
Vector typeMammalian Expression, Retroviral
/ Fusion Protein
- -BioID2-HA (C terminal on backbone)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE 3' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MCS-BioID2-HA pBabe-puro was a gift from Kyle Roux (Addgene plasmid # 120308 ; http://n2t.net/addgene:120308 ; RRID:Addgene_120308)