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Purposeenables eukaryotic expression of human plasma fibronectin
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Depositing Lab
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Depositing OrganizationUniversitaet Heidelberg
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 120401 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 120401-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 120401-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepMAX-Cloning
- Backbone size w/o insert (bp) 2868
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFibronectin
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Alt namePlasma fibronectin containing complete variable region
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Alt nameSoluble fibronectin
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SpeciesH. sapiens (human)
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Insert Size (bp)6914
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Entrez GeneFN1 (a.k.a. CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Clone pFN sp_1. It was used to study the role of the various fibronectin isoforms in transfected eucaryotic cells and to produce the isoforms.
See also "Fibronectins containing extradomain A or B enhance osteoblast differentiation via distinct integrins. J Biol Chem. 2017 May 12;292(19):7745-7760." https://www.ncbi.nlm.nih.gov/pubmed/28325836
DNA (Catalog # 120401-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 120401-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Fibronectin-human-plasma in pMAX was a gift from Inaam Nakchbandi (Addgene plasmid # 120401 ; http://n2t.net/addgene:120401 ; RRID:Addgene_120401) -
For your References section:
EDA-Fibronectin Originating from Osteoblasts Inhibits the Immune Response against Cancer. Rossnagl S, Altrock E, Sens C, Kraft S, Rau K, Milsom MD, Giese T, Samstag Y, Nakchbandi IA. PLoS Biol. 2016 Sep 21;14(9):e1002562. doi: 10.1371/journal.pbio.1002562. eCollection 2016 Sep. PBIOLOGY-D-16-00754 [pii] PubMed 27653627
