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5mCUC1 pGreenII0129
(Plasmid #12067)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12067 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 12067-D50 50 μg of DNA in Tris buffer $495
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DNA 12067-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGreenII0129
  • Backbone size w/o insert (bp) 5000
  • Vector type
    plant transformation via Agrobacterium
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CUC1 mutant
  • Alt name
    CUC1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    3700
  • Mutation
    miR-resistant CUC1. Made by introducing five silent mutations within the miR164-complementarity domain of a CUC1 genomic clone.
  • Entrez Gene
    CUC1 (a.k.a. AT3G15170, ANAC054, ATNAC1, Arabidopsis NAC domain containing protein 54, CUP-SHAPED COTYLEDON1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13 forward 20
  • 3′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See "sequence" for full insert sequence. See article for cloning details.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5mCUC1 pGreenII0129 was a gift from David Bartel (Addgene plasmid # 12067 ; http://n2t.net/addgene:12067 ; RRID:Addgene_12067)

  • For your References section:

    MicroRNA regulation of NAC-domain targets is required for proper formation and separation of adjacent embryonic, vegetative, and floral organs. Mallory AC, Dugas DV, Bartel DP, Bartel B. Curr Biol. 2004 Jun 22. 14(12):1035-46. 10.1016/j.cub.2004.06.022 PubMed 15202996
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