p316CUP1-3SGFP.SG
(Plasmid #1207)

• Depositing Lab: Susan Lindquist
• Publication: Liu et al Nature 1999 Aug 5;400(6744):573-6.

Backbone

• Vector backbone: pRS316
• Backbone size w/o insert (bp): 4847
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: sup35 NM domain
• Alt name: sup35
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1936
• GenBank ID: AY553990
• Entrez Gene: SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: GFP cloned into CUP1-NM with SacI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry