PurposeEctopic expression of ZIKV protein
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||120822||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5535
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameNS3 ZIKV
- Promoter CMV
/ Fusion Protein
- V5 (C terminal on backbone)
- Cloning method Gateway Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.2/NS3 was a gift from Jan Rehwinkel (Addgene plasmid # 120822 ; http://n2t.net/addgene:120822 ; RRID:Addgene_120822)
For your References section:Infection with a Brazilian isolate of Zika virus generates RIG-I stimulatory RNA and the viral NS5 protein blocks type I IFN induction and signaling. Hertzog J, Dias Junior AG, Rigby RE, Donald CL, Mayer A, Sezgin E, Song C, Jin B, Hublitz P, Eggeling C, Kohl A, Rehwinkel J. Eur J Immunol. 2018 Mar 23. doi: 10.1002/eji.201847483. 10.1002/eji.201847483 PubMed 29572905