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ER-moxMaple3
(Plasmid #120885)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120885 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 120885-D50 50 μg of DNA in Tris buffer $495
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DNA 120885-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    ER-mRFP
  • Backbone manufacturer
    Addgene plasmid 62236
  • Modifications to backbone
    ER-RFP contains fusions of the bovine prolactin signal sequence, mRFP, and a KDEL ER retention sequence. mRFP was replaced with moxMaple3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    moxMaple3
  • Species
    Synthetic
  • Promoter CMV
  • Tags / Fusion Proteins
    • bovine prolactin signal sequence (N terminal on backbone) (N terminal on backbone)
    • KDEL ER retention sequence (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please Note: Plasmid contains a 72 basepair deletion in the SV40 promoter sequence upstream of NeoR/KanR compared to the ER-mRFP vector backbone. This deletion is not known to affect plasmid function.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ER-moxMaple3 was a gift from Erik Snapp (Addgene plasmid # 120885 ; http://n2t.net/addgene:120885 ; RRID:Addgene_120885)

  • For your References section:

    moxMaple3: a Photoswitchable Fluorescent Protein for PALM and Protein Highlighting in Oxidizing Cellular Environments. Kaberniuk AA, Mohr MA, Verkhusha VV, Snapp EL. Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.1038/s41598-018-32955-5. 10.1038/s41598-018-32955-5 PubMed 30283009
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