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CMV AP-4
(Plasmid #12101)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12101 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 12101-D50 50 μg of DNA in Tris buffer $495
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DNA 12101-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    modified pBluescript SK+
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AP-4
  • Alt name
    AP4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1270
  • Entrez Gene
    TFAP4 (a.k.a. AP-4, bHLHc41)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuI (destroyed during cloning)
  • 3′ cloning site BglI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Diagnostic digest with XhoI and SphI should release a 1.3kb band. See author's map for more information.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV AP-4 was a gift from Robert Tjian (Addgene plasmid # 12101 ; http://n2t.net/addgene:12101 ; RRID:Addgene_12101)

  • For your References section:

    Transcription factor AP-4 contains multiple dimerization domains that regulate dimer specificity. Hu YF, Luscher B, Admon A, Mermod N, Tjian R. Genes Dev. 1990 Oct . 4(10):1741-52. 10.1101/gad.4.10.1741 PubMed 2123466
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