Type I inositol-1,4,5-trisphosphate 5-phosphatase in pcDNA3
(Plasmid
#121976)
-
Purposetransfection studies in eukaryotic cells such as COS-7 cells
-
Depositing Lab
-
Depositing OrganizationUniversite Libre de Bruxelles
Located in Belgium -
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 121976 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 121976-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 121976-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepcDNA3
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameType I Inositol-1,4,5-trisphoosphate 5-phosphatase
-
Alt nameINPP5A
-
SpeciesH. sapiens (human)
-
GenBank IDX77567.1
-
Entrez GeneINPP5A (a.k.a. 5PTASE)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRV (unknown if destroyed)
- 5′ sequencing primer TAATACGACTCAC
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 121976-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 121976-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Type I inositol-1,4,5-trisphosphate 5-phosphatase in pcDNA3 was a gift from Christophe Erneux (Addgene plasmid # 121976 ; http://n2t.net/addgene:121976 ; RRID:Addgene_121976) -
For your References section:
Post-translational modification of human brain type I inositol-1,4,5-trisphosphate 5-phosphatase by farnesylation. De Smedt F, Boom A, Pesesse X, Schiffmann SN, Erneux C. J Biol Chem. 1996 Apr 26;271(17):10419-24. PubMed 8626616
