pRRLSIN.cPPT.PGK.NeuroD2.WPRE
(Plasmid
#122054)
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PurposeLentiviral expression of NeuroD2
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122054 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRRLSIN.cPPT.PGK.WPRE
- Backbone size w/o insert (bp) 6667
- Total vector size (bp) 7819
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNeuroD2
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SpeciesM. musculus (mouse)
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Insert Size (bp)1152
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GenBank IDNM_010895.3
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Entrez GeneNeurod2 (a.k.a. Ndrf, bHLHa1)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer AGTCGGCTCCCTCGTTGACC
- 3′ sequencing primer CAGCGTATCCACATAGCGTAAA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRRLSIN.cPPT.PGK.NeuroD2.WPRE was a gift from Stephen Tapscott (Addgene plasmid # 122054 ; http://n2t.net/addgene:122054 ; RRID:Addgene_122054) -
For your References section:
Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage. Fong AP, Yao Z, Zhong JW, Johnson NM, Farr GH 3rd, Maves L, Tapscott SJ. Cell Rep. 2015 Mar 31;10(12):1937-46. doi: 10.1016/j.celrep.2015.02.055. Epub 2015 Mar 19. 10.1016/j.celrep.2015.02.055 PubMed 25801030
