pIVT/CamKII gamma CA
(Plasmid #122142)

• Purpose: For making constitutively active CamKII gamma RNA
• Depositing Lab: Carmen Williams
• Publication: Miao et al Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4169-74. Epub 2012 Feb 27.

Backbone

• Vector backbone: pIVT
• Backbone manufacturer: Carmen Williams
• Backbone size w/o insert (bp): 3059
• Vector type: For in vitro transcription

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: constitutively active CamKII gamma
• Species: M. musculus (mouse)
• Insert Size (bp): 873
• Mutation: First 291 amino acid residues of CaMKII gamma
• Entrez Gene: Camk2g (a.k.a. Camkg)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Pst1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry