pIVT/Smarca4
(Plasmid #122146)

• Purpose: For making Smarca4 RNA
• Depositing Lab: Carmen Williams
• Publication: Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. doi: 10.1093/biolre/ioy004.

Backbone

• Vector backbone: pIVT2
• Backbone manufacturer: Carmen Williams
• Backbone size w/o insert (bp): 3059
• Modifications to backbone: Mouse Smarca4 inserted between Sal1 and Xba1 in pIVT2.
• Vector type: For in vitro transcription

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Smarca4
• Alt name: Brg1
• Species: M. musculus (mouse)
• Insert Size (bp): 4845
• Entrez Gene: Smarca4 (a.k.a. BAF190A, Brg1, HP1-BP72, SNF2beta, SW1/SNF, b2b508.1Clo, b2b692Clo)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Smarca4 is from Addgene plasmid #25855.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry