pSW104
(Plasmid
#122295)
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PurposeFor run-off transcription of yeast RPL41A mRNA with and without an 82 nucleotide poly(A) tail.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122295 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSP65A
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Modifications to backboneAdded a T7 promoter in front of the RPL41A gene to allow run off in vitro transcription of RPL41A from T7 with or without a polyA tail, depending on whether the vector is cut with BamHI or HindIII.
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Vector typein vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Strain must have F' episome or the plasmid will lose the poly(A) tail. We used TOP10F'.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRPL41A
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)316
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSW104 was a gift from Sarah Walker (Addgene plasmid # 122295 ; http://n2t.net/addgene:122295 ; RRID:Addgene_122295) -
For your References section:
Reconstitution and analyses of RNA interactions with eukaryotic translation initiation factors and ribosomal preinitiation complexes. Liu X, Schuessler PJ, Sahoo A, Walker SE. Methods. 2019 Mar 26. pii: S1046-2023(18)30395-5. doi: 10.1016/j.ymeth.2019.03.024. 10.1016/j.ymeth.2019.03.024 PubMed 30926531
