YSBE415: pTOP_ACA1_Vc_HOG1 terminator
PurposePlasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus protein
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||122434||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typetagging proteins in Cryptococcus neoformans
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
Growth Strain(s)ccdB Survival
Gene/Insert nameC-terminal fragment of Venus protein
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:YSBE415: pTOP_ACA1_Vc_HOG1 terminator was a gift from Yong-Sun Bahn (Addgene plasmid # 122434 ; http://n2t.net/addgene:122434 ; RRID:Addgene_122434)
For your References section:Molecular Characterization of Adenylyl Cyclase Complex Proteins Using Versatile Protein-Tagging Plasmid Systems in Cryptococcus neoformans. So YS, Yang DH, Jung KW, Huh WK, Bahn YS. J Microbiol Biotechnol. 2017 Feb 28;27(2):357-364. doi: 10.4014/jmb.1609.09036. 10.4014/jmb.1609.09036 PubMed 27780958