rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)
(Plasmid #122450)

• Purpose: Expression of inositol 1,4,5-trisphosphate 3-kinase A
• Depositing Lab: Christophe Erneux
• Publication: Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

Backbone

• Vector backbone: pBluescript
• Backbone manufacturer: Stratagene
• Total vector size (bp): 2900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: inositol 1,4,5-trisphosphate 3-kinase A
• Alt name: ITPKA
• Species: R. norvegicus (rat)
• Insert Size (bp): 1600
• Mutation: coding sequence from V66 till stop codon
• GenBank ID: X56917.1
• Entrez Gene: Itpka
• Tag / Fusion Protein:
  • beta-galactosidase (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13 forward primer
• 3′ sequencing primer: M13 reverse primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Addgene NGS unable to fully confirm the length of the poly-A tail on the inositol 1,4,5-trisphosphate 3-kinase A insert sequence. This is not known to affect plasmid function.