pStrep-4xEGFP-N1
(Plasmid #122491)

• Purpose: Expression of Strep-tagged 4xEGFP
• Depositing Lab: Georg Mayr
• Publication: Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 10.1016/j.ab.2019.113430.

Backbone

• Vector backbone: pEGFP-N1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Strep-tagged 4xEGFP
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspEI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: AGCTGGTTTAGTGAACCGTCA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: The MCS of Strep-tagged multimeric EGFP vectors differs from the MCS of the Strep-tagged monomeric EGFP vector.
pStrep-1xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT CGA ATT CTG CAG TCG ACG GTA CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG
pStrep-2x/…/12xEGFP-N1:
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA GCC ACC ATG AGC GCT TGG AGC CAC CCG CAG TTC GAA AAA CGG GAT CCA CCG GTC GCC ACC ATG GTG