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pUF1-dCas9-Sir2a
(Plasmid #122510)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 122510 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUF1
  • Backbone size w/o insert (bp) 11013
  • Vector type
    CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-Sir2a
  • Alt name
    dCas9-HDAC
  • Species
    Plasmodium falciparum
  • Mutation
    dCas9(D10A,H840A)
  • Promoter PfHsp86
  • Tag / Fusion Protein
    • 3*FLAG (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Plasmid contains a A250T mutation in Sir2a. This mutation is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUF1-dCas9-Sir2a was a gift from Lubin Jiang (Addgene plasmid # 122510 ; http://n2t.net/addgene:122510 ; RRID:Addgene_122510)
  • For your References section:

    Epigenetic editing by CRISPR/dCas9 in Plasmodium falciparum. Xiao B, Yin S, Hu Y, Sun M, Wei J, Huang Z, Wen Y, Dai X, Chen H, Mu J, Cui L, Jiang L. Proc Natl Acad Sci U S A. 2019 Jan 2;116(1):255-260. doi: 10.1073/pnas.1813542116. Epub 2018 Dec 24. 10.1073/pnas.1813542116 PubMed 30584102