Human Inositol 1,4,5-Trisphosphate 3-Kinase B
(Plasmid #122589)

• Purpose: Expression of human inositol 1,4,5-trisphosphate 3-kinase B
• Depositing Lab: Christophe Erneux
• Publication: Dewaste et al Biochem Biophys Res Commun. 2002 Feb 22;291(2):400-5. doi: 10.1006/bbrc.2002.6456.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 8435
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: inositol 1,4,5-trisphosphate 3-kinase B
• Alt name: ITPKB
• Species: H. sapiens (human)
• Insert Size (bp): 3007
• GenBank ID: CAB65055.3
• Entrez Gene: ITPKB (a.k.a. IP3-3KB, IP3K, IP3K-B, IP3KB, PIG37)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry