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pASR130
(Plasmid #122638)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 122638 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPEPZ-Plac
  • Backbone manufacturer
    Jan-Willem Veening
  • Backbone size (bp) 4314
  • Modifications to backbone
    Replacement of spectinomycin marker with trimethoprim marker
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Ampicillin marker for selection in E. coli (100 ug/ml) and trimethoprim marker for selection in S. pneumoniae (10 ug/ml)
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pASR130 was a gift from Jan-Willem Veening (Addgene plasmid # 122638 ; http://n2t.net/addgene:122638 ; RRID:Addgene_122638)
  • For your References section:

    Three New Integration Vectors and Fluorescent Proteins for Use in the Opportunistic Human Pathogen Streptococcus pneumoniae. Keller LE, Rueff AS, Kurushima J, Veening JW. Genes (Basel). 2019 May 22;10(5). pii: genes10050394. doi: 10.3390/genes10050394. 10.3390/genes10050394 PubMed 31121970