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pBabe puro H-Ras G12V T35S
(Plasmid #12274)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 12274 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5150
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H-Ras G12V T35S
  • Alt name
    HRas
  • Species
    H. sapiens (human)
  • Mutation
    Contains G12V activating mutation. The T35S mutation allows binding and activation of Raf, but not of PI3K or Ral-GEF.
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, KRAS, KRAS2, Ki-Ras, RASH1, RASK2, c-K-ras, c-Ki-ras, p21ras)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    See Rodriguez-Viciana, P., et al. (1997) Cell 89, pp. 457
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

A BamHI-SalI fragment from the pWZL Blast ras mutants were individually cloned into the pBABE puro vector directionally.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe puro H-Ras G12V T35S was a gift from Bob Weinberg (Addgene plasmid # 12274 ; http://n2t.net/addgene:12274 ; RRID:Addgene_12274)
  • For your References section:

    Species- and cell type-specific requirements for cellular transformation. Rangarajan A, Hong SJ, Gifford A, Weinberg RA. Cancer Cell. 2004 Aug . 6(2):171-83. 10.1016/j.ccr.2004.07.009 PubMed 15324700