pBabe puro H-Ras G12V T35S
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12274||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5150
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameH-Ras G12V T35S
SpeciesH. sapiens (human)
MutationContains G12V activating mutation. The T35S mutation allows binding and activation of Raf, but not of PI3K or Ral-GEF.
Entrez GeneHRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE 3' (Common Sequencing Primers)
A BamHI-SalI fragment from the pWZL Blast ras mutants were individually cloned into the pBABE puro vector directionally.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe puro H-Ras G12V T35S was a gift from Bob Weinberg (Addgene plasmid # 12274 ; http://n2t.net/addgene:12274 ; RRID:Addgene_12274)
For your References section:Species- and cell type-specific requirements for cellular transformation. Rangarajan A, Hong SJ, Gifford A, Weinberg RA. Cancer Cell. 2004 Aug . 6(2):171-83. 10.1016/j.ccr.2004.07.009 PubMed 15324700