pAS1
(Plasmid #12388)

• Depositing Lab: Nick Rhind
• Publication: Sigova et al Genes Dev. 2004 Oct 1. 18(19):2359-67.

Backbone

• Vector backbone: pRIP2
• Backbone size w/o insert (bp): 7090
• Vector type: Yeast Expression
• Selectable markers: ura4

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: green fluorescent protein
• Alt name: GFP
• Species: Aequorea victoria
• Insert Size (bp): 1500
• GenBank ID: AAB02576

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry