pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP
(Plasmid #124066)

• Purpose: E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.
• Depositing Lab: Tom Rapoport
• Publication: Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi: 10.7554/eLife.18605.

Backbone

• Vector backbone: pProEx-Htb
• Backbone manufacturer: Life Technologies
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: cytATL-R232Q-sfGFP
• Alt name: X. laevis cytosolic Atlastin Fragment R232Q inactive mutant
• Alt name: Codon Optimized for E. Coli expression.
• Species: X. laevis (frog)
• Mutation: R232Q
• Promoter: trc
• Tag / Fusion Protein:
  • sfGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: M13_pUC_Rev_primer
• 3′ sequencing primer: pBAD_Rev_primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry