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pcDNA3.1-Orange_CaMBI_110
(Plasmid #124094)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124094 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 124094-D50 50 μg of DNA in Tris buffer $495
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DNA 124094-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 6330
  • Total vector size (bp) 8760
  • Modifications to backbone
    Chicken beta-actin promoter + Chicken beta-actin intron+ rabbit beta-globin intron inserted between CMV enhancer and inserted gene
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Orange CaMBI 110
  • Species
    Synthetic
  • Insert Size (bp)
    2430

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The C-terminal copy of the CyOFP coding sequence is codon diversified to facilitate molecular biology procedures on Orange CaMBI and reduce the possibility of recombination between the two copies of the CyOFP coding sequence.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-Orange_CaMBI_110 was a gift from Michael Lin (Addgene plasmid # 124094 ; http://n2t.net/addgene:124094 ; RRID:Addgene_124094)

  • For your References section:

    An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging. Oh Y, Park Y, Cho JH, Wu H, Paulk NK, Liu LX, Kim N, Kay MA, Wu JC, Lin MZ. Nat Chem Biol. 2019 Apr 1. pii: 10.1038/s41589-019-0256-z. doi: 10.1038/s41589-019-0256-z. 10.1038/s41589-019-0256-z PubMed 30936501
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