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pAAV-UbC-mNeonGreen
(Plasmid #124102)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 124102 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-UbC
  • Backbone manufacturer
    Chung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
  • Backbone size w/o insert (bp) 5162
  • Total vector size (bp) 5897
  • Modifications to backbone
    The anti-luciferase cassette was deleted and eGFP was replaced with mNeonGreen
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mNeonGreen
  • Species
    Synthetic; Branchiostoma lanceolatum
  • Insert Size (bp)
    735
  • Mutation
    Codon usage was optimized for Homo Sapiens
  • GenBank ID
    KC295282.1
  • Promoter UbC

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Common sequencing primers pCAG-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The gene was synthesized to match the Human codon usage.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-UbC-mNeonGreen was a gift from Pantelis Tsoulfas (Addgene plasmid # 124102 ; http://n2t.net/addgene:124102 ; RRID:Addgene_124102)