Rae1-pDONR221
(Plasmid
#124240)
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PurposeGateway entry clone of Drosophila Rae1 cDNA.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124240 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR221
- Backbone size w/o insert (bp) 2563
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDrosophila Rae1
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Alt namedRae1
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Alt nameCG9862
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SpeciesD. melanogaster (fly)
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Insert Size (bp)1038
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Entrez GeneRae1 (a.k.a. Dmel_CG9862, CG9862, Dmel\CG9862, dmrae1, rae1)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDGC Gold cDNA collection, BDGP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rae1-pDONR221 was a gift from Peter Vilmos (Addgene plasmid # 124240 ; http://n2t.net/addgene:124240 ; RRID:Addgene_124240) -
For your References section:
The actin binding cytoskeletal protein Moesin is involved in nuclear mRNA export. Kristo I, Bajusz C, Borsos BN, Pankotai T, Dopie J, Jankovics F, Vartiainen MK, Erdelyi M, Vilmos P. Biochim Biophys Acta Mol Cell Res. 2017 Oct;1864(10):1589-1604. doi: 10.1016/j.bbamcr.2017.05.020. Epub 2017 May 27. 10.1016/j.bbamcr.2017.05.020 PubMed 28554770
