Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12436||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerBruce Mayer
- Backbone size w/o insert (bp) 6100
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namebZIP of C/EBPa
SpeciesH. sapiens (human)
MutationpEBG vector is from Bruce Mayer, may be different from other pEBG vectors in public domain.
Entrez GeneCEBPA (a.k.a. C/EBP-alpha, CEBP)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
bZIP of human C/EBPa was PCRed using GGAGCGGGCAAGGC and TCACGCGCAGTTGCCC and inserted into BamHI and EcoRI sites of pEBG vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEBG-bZIPa was a gift from Dong-Er Zhang (Addgene plasmid # 12436 ; http://n2t.net/addgene:12436 ; RRID:Addgene_12436)
For your References section:CCAAT enhancer-binding protein (C/EBP) and AML1 (CBF alpha2) synergistically activate the macrophage colony-stimulating factor receptor promoter. Zhang DE, Hetherington CJ, Meyers S, Rhoades KL, Larson CJ, Chen HM, Hiebert SW, Tenen DG. Mol Cell Biol. 1996 Mar . 16(3):1231-40. PubMed 8622667