pBeta-lacZ-attBx2
(Plasmid #124445)

• Purpose: (Empty Backbone) Plasmid for in vivo enhancer reporter assay. Plasmid contains a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by attB sites.
• Depositing Lab: Yingguang Frank Chan
• Publication: Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554/eLife.42014.

Backbone

• Vector backbone: pBR322
• Backbone manufacturer: Sigma
• Backbone size (bp): 4361
• Modifications to backbone: Added a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by double attB sites."
• Vector type: Mammalian Expression ; Enhancer reporter assay construct
• Promoter: beta globin minimal promoter
• Tag / Fusion Protein:
  • LacZ

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Plasmid created by PhD. Mirna Marinic , at the Chan Lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry