5228 pG-N795
(Plasmid
#1246)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1246 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepG1
- Backbone size w/o insert (bp) 7400
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Unknown
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGlucocorticoid receptor
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Speciesn/a
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site hind111 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJohn Glover
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional Reference: Glover JR and Lindquist S. 1998. Hsp104, Hsp70, and Hsp40: a novel chaperone system that rescues previously aggregated proteins.
Cell Jul 10;94(1):73-82.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
5228 pG-N795 was a gift from Susan Lindquist (Addgene plasmid # 1246) -
For your References section:
Reduced levels of hsp90 compromise steroid receptor action in vivo. Picard D, Khursheed B, Garabedian MJ, Fortin MG, Lindquist S, Yamamoto KR. Nature 1990 Nov 8;348(6297):166-8. 10.1038/348166a0 PubMed 2234079