Cytosolic Fyn FRET Biosensor
(Plasmid
#124654)
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PurposeFRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPet
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Depositing Lab
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Depositing OrganizationUniversity of California, San Diego (UCSD)
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124654 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 124654-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 124654-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCAGGS
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Backbone manufacturerMichiyuki Matsuda lab at Tokyo University / Addgene
- Backbone size w/o insert (bp) 4739
- Total vector size (bp) 6521
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Modifications to backboneSal I in the backbone was mutated.
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSynthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
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Alt nameCytosolic Fyn Biosensor
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Insert Size (bp)1782
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 124654-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 124654-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Cytosolic Fyn FRET Biosensor was a gift from Yingxiao Wang (Addgene plasmid # 124654 ; http://n2t.net/addgene:124654 ; RRID:Addgene_124654) -
For your References section:
Sensitive FRET Biosensor Reveals Fyn Kinase Regulation by Submembrane Localization. Ouyang M, Wan R, Qin Q, Peng Q, Wang P, Wu J, Allen M, Shi Y, Laub S, Deng L, Lu S, Wang Y. ACS Sens. 2019 Jan 25;4(1):76-86. doi: 10.1021/acssensors.8b00896. Epub 2019 Jan 9. 10.1021/acssensors.8b00896 PubMed 30588803
