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PH-Btk-mRuby2
(Plasmid #124657)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124657 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 124657-D50 50 μg of DNA in Tris buffer $495
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DNA 124657-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pmRuby2-N1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BTK
  • Alt name
    PH domain of Bruton tyrosine kinase (aa 1–177)
  • Species
    H. sapiens (human)
  • Mutation
    contains only the PH domain of Bruton tyrosine kinase (aa 1–177)
  • GenBank ID
    NM_000061.2
  • Entrez Gene
    BTK (a.k.a. AGMX1, AT, ATK, BPK, IGHD3, IMD1, PSCTK1, XLA)
  • Tag / Fusion Protein
    • mRuby2 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PH-Btk-mRuby2 was a gift from Cheng-han Yu (Addgene plasmid # 124657 ; http://n2t.net/addgene:124657 ; RRID:Addgene_124657)

  • For your References section:

    Tail domains of myosin-1e regulate phosphatidylinositol signaling and F-actin polymerization at the ventral layer of podosomes. Zhang Y, Cao F, Zhou Y, Feng Z, Sit B, Krendel M, Yu CH. Mol Biol Cell. 2019 Mar 1;30(5):622-635. doi: 10.1091/mbc.E18-06-0398. Epub 2019 Jan 2. 10.1091/mbc.E18-06-0398 PubMed 30601698
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