PurposeExpress human LDLR under human APOAI promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||124964||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 24000
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namehuman LDLR
SpeciesH. sapiens (human)
Entrez GeneLDLR (a.k.a. FH, FHC, FHCL1, LDLCQ2)
- Promoter human APOAI
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
As this plasmid is very large, NGS had difficulty sequencing it. There are several differences between Addgene's NGS and depositors sequence. We recommend running additional diagnostic tests before use.
Depositor also recommends growing plasmid at 30 degrees C due to their size.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pD21-AI-hLDLR was a gift from Kazuhiro Oka (Addgene plasmid # 124964 ; http://n2t.net/addgene:124964 ; RRID:Addgene_124964)
For your References section:Gene Therapy Targeting LDL Cholesterol but not HDL Cholesterol Induces Regression of Advanced Atherosclerosis in a Mouse Model of Familial Hypercholesterolemia. Li R, Chao H, Ko KW, Cormier S, Dieker C, Nour EA, Wang S, Chan L, Oka K. J Genet Syndr Gene Ther. 2011 Sep 28;2:106. doi: 10.4172/2157-7412.1000106. 10.4172/2157-7412.1000106 PubMed 23106034