pD21-AI-hLDLR
(Plasmid #124964)

• Purpose: Express human LDLR under human APOAI promoter
• Depositing Lab: Kazuhiro Oka
• Publication: Li et al J Genet Syndr Gene Ther. 2011 Sep 28;2:106. doi: 10.4172/2157-7412.1000106.

Backbone

• Vector backbone: pD21
• Backbone manufacturer: BCM
• Backbone size w/o insert (bp): 24000
• Vector type: Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human LDLR
• Species: H. sapiens (human)
• Entrez Gene: LDLR (a.k.a. FH, FHC, FHCL1, LDLCQ2)
• Promoter: human APOAI

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • #5.pD21-AI-LDLR.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

As this plasmid is very large, NGS had difficulty sequencing it. There are several differences between Addgene's NGS and depositors sequence. We recommend running additional diagnostic tests before use.

Depositor also recommends growing plasmid at 30 degrees C due to their size.