pD21-AI-dsRed
(Plasmid
#124977)
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PurposeExpress dsRed in the liver
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124977 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepD21
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Backbone manufacturerBCM
- Backbone size w/o insert (bp) 24000
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Vector typeAdenoviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedsRed
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SpeciesSynthetic
- Promoter human APOAI
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (unknown if destroyed)
- 3′ cloning site AscI (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
As this plasmid is very large, NGS had difficulty sequencing it. There are several differences between Addgene's NGS and depositors sequence. We recommend running additional diagnostic tests before use.
Depositor also recommends growing plasmid at 30 degrees C due to their size.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pD21-AI-dsRed was a gift from Kazuhiro Oka (Addgene plasmid # 124977 ; http://n2t.net/addgene:124977 ; RRID:Addgene_124977)
